Rat Thyroid-Peroxidase,TPO ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠甲狀腺過氧化物酶(TPO)酶聯(lián)免疫試劑盒（CSB-E08352r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TPO含量。TPO即血小板生成素，其作為造血調(diào)控因子，在血小板生成過程中有關(guān)鍵作用。研究機(jī)制主要圍繞它結(jié)合并激活跨膜TPO受體，啟動信號傳導(dǎo)途徑，促進(jìn)造血干細(xì)胞向巨核系祖細(xì)胞分化、增殖及成熟，進(jìn)而產(chǎn)生血小板。試劑盒檢測范圍為2.5 ng/mL-40ng/mL，適用于甲狀腺相關(guān)疾病的動物模型研究、環(huán)境毒理評估中甲狀腺功能干擾物的篩選，以及甲狀腺自身抗體作用機(jī)制等基礎(chǔ)科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
縮寫：

TPO
Uniprot No.：

P14650
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue Homogenates
檢測范圍：

2.5 ng/mL-40ng/mL
靈敏度：

2.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	95
	Range %	84-99
1:4	Average %	90
	Range %	82-100
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rat TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-102
EDTA plasma (n=4)	94	89-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Peroxidase family, XPO subfamily
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000006526

UniGene: Rn.233736